Search Reference Genes
Search by species name, Latin name, gene name, or family to retrieve reference gene information
| Species ⇅ | Latin Name ⇅ | Family ⇅ | Genes ⇅ | Year ⇅ | Representative Publication |
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About This Database
📚 Data Sources
This database compiles experimentally validated qRT-PCR reference gene information for medicinal plants from published literature, including peer-reviewed journal articles and graduate theses in both Chinese and English.
Each record includes: gene name, primer sequences, Tm values, amplicon length, experimental conditions, screening methods, and original publication details.
🔬 Screening Methods
Reference gene stability is evaluated using multiple algorithms:
- geNorm — pairwise variation analysis
- NormFinder — ANOVA-based model
- BestKeeper — standard deviation & correlation coefficients
- Delta Ct — ΔCt comparison
- RefFinder — comprehensive ranking tool
📊 Coverage
💡 User Guide
- Search: enter species name, gene name, or family to find reference genes
- Browse: view a summary of all cataloged species
- Click a species name in the table to view all its reference gene details
- Click column headers to sort the table